seqbench.com

seqbench.com: enzyme on golden_gate_from_parts narrowed to a closed enum (BsaI, BsmBI, BbsI, SapI); previously valid values may now be rejected.

[risky] Optional field mergePreAddedArms was added to cloning_simulate; may shift model behaviour. [safe] Description of primer_specificity changed (13% word delta). [risky] Optional field intendedTemplate was added to primer_specificity…

• risky Optional field mergePreAddedArms was added to cloning_simulate; may shift model behaviour.
• breaking enzyme on golden_gate_from_parts narrowed to a closed enum (BsaI, BsmBI, BbsI, SapI); previously valid values may now be rejected.
• safe Description of primer_specificity changed (13% word delta).
• risky Optional field intendedTemplate was added to primer_specificity; may shift model behaviour.
• risky Description of verify_assembly changed (38% word delta).
• risky Optional field dephosphorylateVector was added to verify_assembly; may shift model behaviour.
• risky Optional field mergePreAddedArms was added to verify_assembly; may shift model behaviour.
• risky Optional field vectorEnzyme3 was added to verify_assembly; may shift model behaviour.

+ 2 more in this revision

• Description of primer_specificity changed (18% word delta).

• Tool golden_gate_from_parts was added.

• risky Description of in_silico_pcr changed (75% word delta).
• safe Description of primer_specificity changed (17% word delta).

• Tool ligation_setup was added.

• safe Tool assembly_outcomes was added.
• safe Tool band_traceback was added.
• safe Tool diagnostic_digest was added.
• safe Tool multiplex_panel_design was added.
• safe Tool repeat_instability was added.
• safe Tool sanger_indel_spectrum was added.
• risky Description of primer_specificity changed (27% word delta).

• Tool prime_editing_efficiency was added.

• safe Tool oligo_cofold was added.
• risky Description of crispr_grna_design changed (78% word delta).
• risky Description of crispr_offtarget_check changed (67% word delta).
• risky Description of rbs_design changed (45% word delta).
• risky Description of rbs_predict changed (48% word delta).
• risky Description of rna_fold changed (43% word delta).
• risky Description of sirna_design changed (29% word delta).